Southern blot - Wikipedia
A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.. The method is named after the British biologist Edwin Southern, who first published it in 1975.
Southern Blotting | Thermo Fisher Scientific - US
Southern blot analysis reveals information about DNA identity, size, and abundance. It is a classic technique that involves separating DNA fragments based on size via electrophoresis, transferring them to a membrane, hybridization with a labeled sequence-specific probe, washing, and finally ...
Southern Blotting Technique | Ask A Biologist
Southern Blotting. Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1970s. To oversimplify, DNA molecules are transferred from an agarose gel onto a membrane. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture.
Southern Blotting: principle, procedure and application ...
Southern Blotting: principle, procedure and application Principle: Southern blotting is an example of RFLP (restriction fragment length polymorphism). It was developed by Edward M. Southern (1975). Southern blotting is a hybridization technique for identification of particular size of DNA from the mixture of other similar molecules.
Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. The method is named after its inventor ...
Southern blot (video) | DNA technology | Khan Academy
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Southern Blotting Principle, Procedure and Application ...
Southern blotting. The technique was developed by E.M. Southern in 1975. The Southern blot is used to detect the presence of a particular DNA fragment in a sample. The DNA detected can be a single gene, or it can be part of a larger piece of DNA such as a viral genome. Principle The key to this method is hybridization.