Genome Assembly - an overview | ScienceDirect Topics
A genome assembly is simply the sequence produced after all of the chromosomes of a target species have been fragmented (a large number of short/long DNA sequences), sequenced, and computationally put back together again to create a representation of the original intact chromosome sequences.

De novo genome assembly: what every biologist should know
Genome assembly stitches together a genome from short sequenced pieces of DNA. Credit: Michael Schatz, Cold Spring Harbor. Paired reads typically are about the same length as...

Genome assembly in the telomere-to-telomere era - Nature
Here, we review recent progress on assembly algorithms and protocols, with a focus on how to derive near-telomere-to-telomere assemblies. We also discuss the additional developments...

Sequence assembly - Wikipedia
As the sequenced organisms grew in size and complexity (from small viruses over plasmids to bacteria and finally eukaryotes), the assembly programs used in these genome projects needed increasingly sophisticated strategies to handle:

Ten steps to get started in Genome Assembly and Annotation
With these 10 steps we aim to fill this need. In a de novo genome assembly and annotation project, the nucleotide sequence of a genome is first assembled, as completely as possible, and then annotated. The annotation process infers the structure and function of the assembled sequences.

An Overview of Genome Assembly - CD Genomics
There are two different types of genome assembly: de novo assembly and mapping to a reference genome (also known as reference-based alignment). De novo assembly refers to the genome assembly of a novel genome from scratch without the aid of reference genomic data.

Lecture 6: Genome Assembly - MIT OpenCourseWare
Formulating the assembly problem Finding overlaps is important, and we’ll return to it, but our ultimate goal is to recreate (assemble) the genome How do we formulate this problem? First attempt: the shortest common superstring (SCS) problem http://www.langmead-lab.org/teaching-materials/

Understanding Genome Assembly Using De Bruijn Graphs: From Concepts to ...
This project demonstrates the fundamental concepts behind genome assembly using De Bruijn graphs and Eulerian paths. By simulating reads from a random DNA sequence and applying graph algorithms, we can piece together the original genome sequence.

Next-Generation Sequence Assembly: Four Stages of Data Processing and ...
In this review, we address the basic framework of next-generation genome sequence assemblers, which comprises four basic stages: preprocessing filtering, a graph construction process, a graph simplification process, and postprocessing filtering.

Genome Assembly - SpringerLink
Genome assembly is the computational process of deciphering the sequence composition of the genetic material (DNA) within the cell of an organism, using numerous short sequences called reads derived from different portions of the target DNA as input.